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UTILIZATION OF NON-ALCOHOLIC EXTRACT OF PIGMENT DERIVED FROM LOCAL ROSE AS ACTIVE NATURAL COLORANT TO PROVIDE A SAFE AND HALAL PRODUCT (Seminar Seminar Internasional, Jakarta International Conference of Muslim Intellectuals (JICMI) 2013, 14-15 Desember

Elfi Anis Saati, Rokhmatul Aisyah, Muhammad Wachid, Sri Winarsih

Abstract


Rose is a well-known cut ornamental flower mostly cultivated in Batu city, Indonesia. The crown of local roses from Batu have been investigated to have high anthocyanin content, in particular for the type of Cyanidine and Dephinidine-glycoside (Saati, et, al,. 2007) as well as Malvidine-glycoside (Saati, 2011). Those bioactive compounds have been proven to act as free-radical scavenger. Low grade and non-fresh flowers still contains potential pigments which can be utilized as natural colorant for food and supplement such as effervescent tablet. The aims of this study were to investigate the effect of post-cutting process on the quality of anthocyanin extract of local roses, to investigate the effect of rose anthocyanin on the quality of three different products, and to examine bioactivity of rose effervescent tables on the liver and kidney protection through SGPT-SGOT test using white rat.This study was carried out into three  experiments. First experiment was evaluation of quality of rose pigment extract in three different conditions, i.e. fresh cut-rose, 2 days after cutting, and 4 days after cutting, which was conducted using Randomized Block Design (RBD). Second experiment was application of rose pigment extract to some industrial product, i.e. food, herbal products, using designs in accordance with its expected targets. The end step was studied bioactive test using white rat. Results showed that 2-4% of rose anthocyanin not only markedly contributed in natural red and yellow color of several industrial products such as fruit juices, jelly, and carbonated drink, but also was proven to exhibit strong bioactivity. One Way ANOVA and Post Tukey HSD Test indicated that administration of 1 effervescent tablet containing 4.85 mg of anthocyanin, significantly decreased MDA, SGPT, and SGOT levels, from 0.25±0.02 mg/dL (treated with CCl4) to 0.06±0.01 mg/dL; from 84.50±10.91 to 27.80±3.29; and from 113.89±12.57 to 43.68±5.44, respectively. 

Keywords


Key words: anthocyanin, bioactive product, local roses, natural colorant

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