Identification and Patogenisity Test of Fusarium spp. That Caused Crown Rot of Moth Orchid (Phalaenopsis sp.)

Khaterine Khaterine, Rina Sri Kasiamdari


Abstract Moth orchid is one of extensively cultivated orchid in Indonesia. In its cultivation, crown rot is one of diseases that usually attack moth orchid. This research aimed to identifiedFusarium that caused crown rot on moth orchid. This research expected can give useful information to improve methods of prevent and against crown rot on moth orchid. Pathogenic fungi was isolated from Phalaenopsis Kung Valentine that infected by crown rot. Obtained isolates than tested for its pathogenicity to P. amabilis healty leaves in vitro. The most pathogenic isolate then identified to know its spesies. Result showed that eight Fusarium were isolated. Then the isolates were grouped in 3 group that were Fusarium sp. isolate 1A, Fusarium sp.isolate 2A dan Fusarium sp. isolate 4A. Pathogenicity test result showed no symptom was observed on control, Fusarium sp. isolate 1A was weakly pathogenic (1,67), whereas Fusarium sp. isolate 2A andFusarium sp. isolate 4A showed same pathogenicity rating with score 3. Even had the same pathogenicity rating, but Fusarium sp. isolate 4A could be indicated as the mainĀ pathogen that caused crown rot on moth orchid, based on its diseases progression speed, and it was the most frequently isolated isolate in this research. Based on its identification, Fusarium sp. isolate 4A is Fusarium oxysporum.


Moth orchid; crown rot

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